Cmmb Notes

05-09-13

CMMB 511

No final exam

Journal based course.

Try to think of everything in terms of GENE EXPRESSION

Biol questions

1) concepts

2) strategies and approaches – need cross f(x) or complimentary approaches to develop a compreshensive understanding

3) techniques – lots of techniques, make sure using appropriate techniques

4) outcome of results

5) overall conclusions

DNA content per haploid genome:

Bacteria: 2 x 106 bp

Mammal: 2 x 109 bp

Complexity of the genomes. Important question in biol scinces is

1) How is the genome organized?

2) How is gene expression regulated? Very tightly regulated, don’t expect genes to go up or doen. Different mechanisms, remarkable complexity.

These are important questions in the understanding of cellular processes including:

cell differentiation, development, diseases (etc). the study of processes comes down to the two above questions. If you know the basic concepts, can answer anything!

05-09-15

How is the genome organized?

How do we know the presence of the sequences. Will re-visit classic methods to determine the sequence of these genomes.

S(X) of the chromo

Each chromo is a single, linear DNA molecule

At the