Extraction of Dna from Calf or Hog Thymus/isolation of Yeast Rna

Extraction Of Dna From Calf Or Hog Thymus/Isolation Of Yeast Rna

Submitted by wasabi on July 5, 2006

Category: Science

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Extraction of DNA from Calf or Hog Thymus/Isolation of Yeast RNA

I. Abstract

Nucleic acids may be divided into two groups RNA and DNA. DNA contains almost all the genetic information while RNA serves as the bridge between the DNA and proteins.

Study of both DNA and RNA initially involves proper extraction/isolation. The storehouse of eukaryotic DNA is the nucleus (and in the mitochondria), so experimentally, DNA is extracted from tissues that have a high nuclear to cytoplasmic mass ratio, such as the tissues of the thymus gland and spleen. The thymus gland is a particularly good site for DNA extraction because it functions as the primary site for T lymphocyte differentiation. The T lymphocytes it acts upon have a round nucleus that occupies a greater proportion of the cellпїЅпїЅs volume (with only a thin layer of cytoplasm surrounding the nucleus). The high lymphocyte content of the thymus gland, makes DNA extraction much more