Shrimp Lab

Jacob Veit

10/21/2016

1

Pre-Lab Questions:

1.

Speciation: the formation of new and distinct species in the course of evolution.

Mutation: the action or process of mutating.

Cladogram: a branching diagram showing the cladistic relationship between a number of species.

2.  How an organism changes due to an external force.  Because a polar bear's fur looks white, for example, it can blend in with the snow.

1. Title:  The Brine Shrimp Hatchery Lab

1. Objective:

To observe how three different salt-water solutions(0.5%, 1.0%, and 1.5%) sustain brine shrimp eggs.

1. Hypothesis:

If brine shrimp eggs are placed in three different salt-water solutions (0.5%, 1.0%, and 1.5), then one will be able to record the differences of how each solution sustains the brine shrimp eggs.

1. Materials:

• Brine shrimp eggs
• Scissors
• 5 pipets
• Paintbrush
• 5 microscope slides
• 1 microscope
• Double-sided tape
• Distilled water
• 5 petri dishes
• Permanent marker
• Graduated cylinder
• Paper towels
• 5 beekers
• Sodium chloride

1. Procedure:

Day 1:

1.  Prepare and label 3 30-ml salt solutions, using sodium chloride and distilled water. The solutions should be 0.5%, 1.0% and 1.5% NaCl. Use the space in the analysis section that follows to determine what mass of sodium chloride to add to each solution.
2.  Label three petri dishes 0.5%, 1.0%, and 1.5%.
3.  Use a graduated cylinder to measure 30ml of each solution and pour them into their corresponding petri dishes.
4.  Measure and cut 1.5cm of double-sided tape.
5.  Stick the double-sided tape to a microscope slide.
6.  Lightly touch the paintbrush to the side of the bag containing the brine shrimp eggs. Your goal is to collect only approximately 20 eggs on the brush. You do not want to cover the tip of the brush in eggs.
7.  Dab the paintbrush onto the tape on the microscope slide.
8.  Examine the slide under a microscope.
9.  Count the number of eggs on the slide and record this number in table 1 under 0 hours.
10.  Place the microscope slide in the 0.5% petri dish, tape-side up,and place the lid on the dish.
11.  Follow steps 4-10 for the remaining slides and dishes, until you have prepared  three microscope slides of eggs, recorded the numbers in table 1, and placed each slide in its appropriate salt solution.
12.  Allow the dishes to sit  room temperature undisturbed for 24 hours.

Day 2:

1.  Examine one petri dish with a microscope
2.  Count the number of swimming brine shrimp. With a pipet, gently catch them and move them into another container. Try not to remove excess water with the pipet.
3.  Record the number of swimming shrimp in table 1 under 24 hours.
4.  Count the number of dead or partially hatched shrimp and record this number in table 1 under 24 hours.
5.  Count the number of unhatched eggs and record this number in table 1 under 24 hours.
6.  Repeat steps 1-5 for each of the petri dishes

Day 3:

1.  Examine one petri dish with a microscope
2.  Count the number of swimming brine shrimp. With a pipet, gently catch them and move them into another container. Try not to remove excess water with the pipet.
3.  -record the number of swimming shrimp in table 1 under 48 hours.
4.  Count the number of dead or partially hatched shrimp and record this number in table 1 under 48 hours.
5.  Count the number of unhatched eggs and record this number in table 1 under 48 hours.
6. Repeat steps 1-5 for each of the petri dishes

Data/Graphs:

[pic 1]

1. Calculations:

No calculations were made.

Post-Lab Questions:

1.the 1.0% solution had the most hatching going on. I was incorrect tin my prediction because I had thought that the 1.5% solution would have had the most hatching of eggs.

2.the 1.0% solution had the most hatching because the solution most-likely had just the right amount of salt and water to sustain the shrimp.

3.two variables include whether the shrimp hatched or not and how the shrimp interacted with each other.