Lobsters Are Osmoregulators

Methods

To determine the principals of osmoregulation, we sampled two lobsters from each tank and there were three different tanks which the water ranged in salinity. The experiment is to determine whether the six lobsters tested are osmoregulators or osmoconformers, this is done by obtaining a sample of hemolymph. The first step of the lab is to prepare the needle and syringe that will be taking the hemolymph. The syringe size was 1 ml, and the intention is to collect between 0.5 and 1.0 ml of hemolymph. The needle size was 20 gauge, because anything smaller would destroy the hemolymph cells. Then the lobster was picked up with a firm grim around the dorsal celphao-thorax region and flipped over to expose the ventral side. The hemolymph was be extracted from the central midline of the ventral pre-branchial region, of the first section. Although, before piercing the membrane, the bevel of the needle had to be pointing up. When the needle was injected into the membrane, it did not have to go any deeper than 2-3 mm into the hemocyannin (blood cavity). If the needle went to deep