Micro Boophilius Abstract

Bacterial diversity in the Rhipicephalus Microplus

Renato Andreotti, Adalberto A Pérez de León, Scot E Dowd, Felix D Guerrero, Kylie G Bendele, and Glen A Scoles. Research and Testing Laboratory, Pathogenius, and Spirostat Technologies, Lubbock, TX, Janurary, 6 2011.

The purpose of this study was to illustrate the R. microplus bacterial diversity. Adult male and female ticks were obtained from a R. microplus infestation outbreak on cattle, where the samples were frozen at -80°C. Prior to freezing, eggs were collected from females, mixed together, and labeled as f1 generation. Two adult females and two adult males developed from these f1 eggs and three small clumps of approximately 100 f1 eggs. The tissues were frozen at -80°C. A clump of the frozen material was broken off and used for pyrosequencing analysis. Amplicon sequencing was performed based upon the manufacturer's protocols for Titanium sequencing on FLX-titanium platform. A total of 183,626 sequences were generated and a total of 130,019 sequences utilized for analyses of the